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Biblioteca(s): |
Embrapa Suínos e Aves. |
Data corrente: |
11/05/2021 |
Data da última atualização: |
11/05/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SERIBELLI, A. A.; SILVA, P. da; CRUZ, M. F. da; ALMEIDA, F. de; FRAZÃO, M. R.; MEDEIROS, M. I. C.; RODRIGUES, D. dos P.; KICH, J. D.; BENEVIDES, L. de J.; SOARES, S. de C.; ALLARD, M. W.; FALCÃO, J. P. |
Afiliação: |
AMANDA APARECIDA SERIBELLI, USP; PATRICK DA SILVA, UNESP; MARCELO FERREIRA DA CRUZ, USP; FERNANDA DE ALMEIDA, USP; MILIANE RODRIGUES FRAZÃO, USP; MARTA I. C. MEDEIROS, Instituto Adolfo Lutz; DÁLIA DOS PRAZERES RODRIGUES, FIOCRUZ; JALUSA DEON KICH, CNPSA; LEANDRO DE JESUS BENEVIDES, UFTM; SIOMAR DE C. SOARES, UFTM; MARC WILLIAN ALLARD, Food and Drug Administration; JULIANA PFRIMER FALCÃO, USP. |
Título: |
Insights about the epidemiology of Salmonella typhimurium isolates from different sources in Brazil using comparative genomics. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Gut Pathogens, v. 13, n. 27, 2021. |
DOI: |
https://doi.org/10.1186/s13099-021-00423-7 |
Idioma: |
Inglês |
Conteúdo: |
Abstract Background: Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium) is an important zoonotic agent worldwide. The aim of this work was to compare genetically 117 S. Typhimurium isolated from different sources over 30 years in Brazil using different genomics strategies. Results: The majority of the 117 S. Typhimurium strains studied were grouped into a single cluster (≅ 90%) by the core genome multilocus sequence typing and (≅ 77%) by single copy marker genes. The phylogenetic analysis based on single nucleotide polymorphism (SNP) grouped most strains from humans into a single cluster (≅ 93%), while the strains isolated from food and swine were alocated into three clusters. The different orthologous protein clusters found for some S. Typhimurium isolated from humans and food are involved in metabolic and regulatory processes. For 26 isolates from swine the sequence types (ST) 19 and ST1921 were the most prevalent ones, and the ST14, ST64, ST516 and ST639 were also detected. Previous results typed the 91 S. Typhimurium isolates from humans and foods as ST19, ST313, ST1921, ST3343 and ST1649. The main prophages detected were: Gifsy-2 in 79 (67.5%) and Gifsy-1 in 63 (54%) strains. All of the S. Typhimurium isolates contained the acrA, acrB, macA, macB, mdtK, emrA, emrB, emrR and tolC efflux pump genes. Conclusions: The phylogenetic trees grouped the majority of the S. Typhimurium isolates from humans into a single cluster suggesting that there is one prevalent subtype in Brazil. Regarding strains isolated from food and swine, the SNPs’ results suggested the circulation of more than one subtype over 30 years in this country. The orthologous protein clusters analysis revealed unique genes in the strains studied mainly related to bacterial metabolism. S. Typhimurium strains from swine showed greater diversity of STs and prophages in comparison to strains isolated from humans and foods. The pathogenic potential of S. Typhimurium strains was corroborated by the presence of exclusive prophages of this serovar involved in its virulence. The high number of resistance genes related to efflux pumps is worrying and may lead to therapeutic failures when clinical treatment is needed. MenosAbstract Background: Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium) is an important zoonotic agent worldwide. The aim of this work was to compare genetically 117 S. Typhimurium isolated from different sources over 30 years in Brazil using different genomics strategies. Results: The majority of the 117 S. Typhimurium strains studied were grouped into a single cluster (≅ 90%) by the core genome multilocus sequence typing and (≅ 77%) by single copy marker genes. The phylogenetic analysis based on single nucleotide polymorphism (SNP) grouped most strains from humans into a single cluster (≅ 93%), while the strains isolated from food and swine were alocated into three clusters. The different orthologous protein clusters found for some S. Typhimurium isolated from humans and food are involved in metabolic and regulatory processes. For 26 isolates from swine the sequence types (ST) 19 and ST1921 were the most prevalent ones, and the ST14, ST64, ST516 and ST639 were also detected. Previous results typed the 91 S. Typhimurium isolates from humans and foods as ST19, ST313, ST1921, ST3343 and ST1649. The main prophages detected were: Gifsy-2 in 79 (67.5%) and Gifsy-1 in 63 (54%) strains. All of the S. Typhimurium isolates contained the acrA, acrB, macA, macB, mdtK, emrA, emrB, emrR and tolC efflux pump genes. Conclusions: The phylogenetic trees grouped the majority of the S. Typhimurium isolates from humans into a single cluster suggesting that there is one pr... Mostrar Tudo |
Palavras-Chave: |
Aglomerados ortólogos de proteínas; Árvores filogenéticas; Bombas de efluxo; Efflux pumps; Phylogenetic trees; Profagos; Prophages; Protein orthologous clusters. |
Thesagro: |
Salmonella Typhimurium. |
Categoria do assunto: |
-- |
Marc: |
LEADER 03391naa a2200373 a 4500 001 2131761 005 2021-05-11 008 2021 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1186/s13099-021-00423-7$2DOI 100 1 $aSERIBELLI, A. A. 245 $aInsights about the epidemiology of Salmonella typhimurium isolates from different sources in Brazil using comparative genomics.$h[electronic resource] 260 $c2021 520 $aAbstract Background: Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium) is an important zoonotic agent worldwide. The aim of this work was to compare genetically 117 S. Typhimurium isolated from different sources over 30 years in Brazil using different genomics strategies. Results: The majority of the 117 S. Typhimurium strains studied were grouped into a single cluster (≅ 90%) by the core genome multilocus sequence typing and (≅ 77%) by single copy marker genes. The phylogenetic analysis based on single nucleotide polymorphism (SNP) grouped most strains from humans into a single cluster (≅ 93%), while the strains isolated from food and swine were alocated into three clusters. The different orthologous protein clusters found for some S. Typhimurium isolated from humans and food are involved in metabolic and regulatory processes. For 26 isolates from swine the sequence types (ST) 19 and ST1921 were the most prevalent ones, and the ST14, ST64, ST516 and ST639 were also detected. Previous results typed the 91 S. Typhimurium isolates from humans and foods as ST19, ST313, ST1921, ST3343 and ST1649. The main prophages detected were: Gifsy-2 in 79 (67.5%) and Gifsy-1 in 63 (54%) strains. All of the S. Typhimurium isolates contained the acrA, acrB, macA, macB, mdtK, emrA, emrB, emrR and tolC efflux pump genes. Conclusions: The phylogenetic trees grouped the majority of the S. Typhimurium isolates from humans into a single cluster suggesting that there is one prevalent subtype in Brazil. Regarding strains isolated from food and swine, the SNPs’ results suggested the circulation of more than one subtype over 30 years in this country. The orthologous protein clusters analysis revealed unique genes in the strains studied mainly related to bacterial metabolism. S. Typhimurium strains from swine showed greater diversity of STs and prophages in comparison to strains isolated from humans and foods. The pathogenic potential of S. Typhimurium strains was corroborated by the presence of exclusive prophages of this serovar involved in its virulence. The high number of resistance genes related to efflux pumps is worrying and may lead to therapeutic failures when clinical treatment is needed. 650 $aSalmonella Typhimurium 653 $aAglomerados ortólogos de proteínas 653 $aÁrvores filogenéticas 653 $aBombas de efluxo 653 $aEfflux pumps 653 $aPhylogenetic trees 653 $aProfagos 653 $aProphages 653 $aProtein orthologous clusters 700 1 $aSILVA, P. da 700 1 $aCRUZ, M. F. da 700 1 $aALMEIDA, F. de 700 1 $aFRAZÃO, M. R. 700 1 $aMEDEIROS, M. I. C. 700 1 $aRODRIGUES, D. dos P. 700 1 $aKICH, J. D. 700 1 $aBENEVIDES, L. de J. 700 1 $aSOARES, S. de C. 700 1 $aALLARD, M. W. 700 1 $aFALCÃO, J. P. 773 $tGut Pathogens$gv. 13, n. 27, 2021.
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Embrapa Suínos e Aves (CNPSA) |
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Registro Completo
Biblioteca(s): |
Embrapa Uva e Vinho. |
Data corrente: |
12/01/2017 |
Data da última atualização: |
15/10/2018 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
MELO, G. W. B. de; SILVA, L. S. da; BRUNETTO, G.; ZALAMENA, J.; ALBARELLO, J. B. |
Afiliação: |
GEORGE WELLINGTON BASTOS DE MELO, CNPUV; Universidade Federal de Santa Maria – UFSM, Centro de Cie?ncias Rurais, Programa de Po?s-Grad. em Cie?ncia do Solo, Santa Maria, Rio Grande do Sul State, Brazil.; GUSTAVO BRUNETTO, 2Universidade Federal de Santa Maria – UFSM, Centro de Cie?ncias Rurais, Programa de Po?s-Grad. em Cie?ncia do Solo, Santa Maria, Rio Grande do Sul State, Brazil.; JOVANI ZALAMENA, 1Empresa Brasileira de Pesquisa Agropecua?ria, Embrapa Uva e Vinho, Bento Gonçalves, Rio Grande do Sul State, Brazil; J.B. Albarello, Empresa Brasileira de Pesquisa Agropecua?ria, Embrapa Uva e Vinho, Bento Gonçalves, Rio Grande do Sul State, Brazil. |
Título: |
Sensitivity of grapevine rootstocks to changes in zinc concentration in the soil. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Acta Horticulturae, v. 1136, p. 201-208, 2016. |
Idioma: |
Inglês |
Conteúdo: |
Abstract The objective of this study was to evaluate the effect of high levels of Zn in soil on productivity and nutritional and root performance of young grapevine rootstocks, as well as to identify variables that are affected by excess Zn. Five rootstocks (SO4, Paulsen 1103, IAC572, IAC313 and 420A) were grown in pots containing soil with the addition of 0, 20, 40, 80 or 160 mg kg-1 of Zn. The rootstocks were pruned at 45 days, discarding the shoot. After re-sprouting, a main branch was maintained and evaluated after 50 days in terms of height, number of leaves, and leaf area; dry matter, concentration and accumulation of Zn in the shoots and roots; and area, olume, length and diameter of the root system. The variables of plant height, leaf area, and dry weight of roots and shoots can be used to evaluate the effect of Zn on the growth of young grapevines. The application of Zn to the soil increased the concentration and total accumulation of Zn in the shoots and roots of the grapevine rootstocks. The volume, surface area, and length of vine roots decreased after applying Zn to the soil, and these variables can be used to evaluate the negative effects of Zn on the vines. The number of leaves and the weighted root diameter are not good indicators of changes in young grapevines caused by high levels of Zn in the soil. |
Palavras-Chave: |
Heavy metal; Soil contamination; Zinc phytotoxicity. |
Thesaurus NAL: |
Root growth; Vineyards. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01969nam a2200217 a 4500 001 2060645 005 2018-10-15 008 2016 bl uuuu u00u1 u #d 100 1 $aMELO, G. W. B. de 245 $aSensitivity of grapevine rootstocks to changes in zinc concentration in the soil.$h[electronic resource] 260 $aActa Horticulturae, v. 1136, p. 201-208$c2016 520 $aAbstract The objective of this study was to evaluate the effect of high levels of Zn in soil on productivity and nutritional and root performance of young grapevine rootstocks, as well as to identify variables that are affected by excess Zn. Five rootstocks (SO4, Paulsen 1103, IAC572, IAC313 and 420A) were grown in pots containing soil with the addition of 0, 20, 40, 80 or 160 mg kg-1 of Zn. The rootstocks were pruned at 45 days, discarding the shoot. After re-sprouting, a main branch was maintained and evaluated after 50 days in terms of height, number of leaves, and leaf area; dry matter, concentration and accumulation of Zn in the shoots and roots; and area, olume, length and diameter of the root system. The variables of plant height, leaf area, and dry weight of roots and shoots can be used to evaluate the effect of Zn on the growth of young grapevines. The application of Zn to the soil increased the concentration and total accumulation of Zn in the shoots and roots of the grapevine rootstocks. The volume, surface area, and length of vine roots decreased after applying Zn to the soil, and these variables can be used to evaluate the negative effects of Zn on the vines. The number of leaves and the weighted root diameter are not good indicators of changes in young grapevines caused by high levels of Zn in the soil. 650 $aRoot growth 650 $aVineyards 653 $aHeavy metal 653 $aSoil contamination 653 $aZinc phytotoxicity 700 1 $aSILVA, L. S. da 700 1 $aBRUNETTO, G. 700 1 $aZALAMENA, J. 700 1 $aALBARELLO, J. B.
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